The S-INFL-ELISA kit is designed to measure human S100A12 protein in serum. S100A12 protein is over-expressed at inflammatory sites and its serum concentrations are correlated with disease stage and activity. This kit is "For Research Use Only, not for use in diagnostic procedures". Related product: F-INFL-ELISA (dosage ELISA of S100A12 in stools)
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- S100A12 serum ELISA
Human S100A12 protein (Calgranulin C, EN-RAGE) in serum - ELISA kit Inflamark
Features
- Working range: 21 ? 650 ng/mL
- Sample: serum (obligatory 1/11predilution) ? 30 ?L
- Analytical sensitivity: 21 ng/mL
- Functional sensitivity: -
Applications
- S100A12 (EN-RAGE or Calgranulin C) is a 10 kDa cytoplasmic protein and belongs to the S100 family of calcium binding pro inflammatory proteins predominantly secreted by granulocytes. When secreted extracelllularly, S100A12 contributes to innate immune response including activation of intracellular signaling aiming to cytokine production and induction of oxidative stress.
- S100A12 could be a biomarker of interest for inflammation processes. Its utility was already described in:
- - Cardiovascular (atherosclerosis...)
- - Rheumatology (Rheumatoid Arthritis…)
- - Nephrology (hemodialysis patients…)
- - Pulmonology (ARDS…)
Assay principle
ELISA
A first monoclonal antibody, immobilized on the microplate, captures the S100A12 proteins contained in the calibrators and samples. After washing, the absorbed proteins are then recognized by a second monoclonal antibody conjugated to HRP (Horse-Radish-Peroxidase). A 1/11 sample predilution is required. 3 step protocol: 16-20h at RT + 2h at RT + 10 min at RT.