The OCFE07-Tryps kit permits radioimmunological determination of the enzyme trypsin in human serum and other biological fluids. The kit utilizes the principle of competitive protein binding analysis, using a double antibody radioimmunoassay method where free trypsin is separated from antibody-bound trypsin by an anti-rabbit precipitating serum.
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- Trypsin RIA
Human trypsin (htrypsin) radioimmunological assay kit
Features
- Working range: 0 ? 1200 ng/mL
- Sample: serum ? 100 ?L
- Analytical sensitivity: 8.0 ng/mL
Applications
- Differential diagnosis in presence of acute abdominal and other symptoms due to pancreatic disease
Assay principle
RIP
Imminoprecipitating reagent
In the first step, serum trypsin and 125I-radiolabelled trypsin compete for highly specific anti-trypsin antibodies. In the second step, the trypsin or 125I-radiolabelled trypsin antibody complex, which reacts with an anti-rabbit gammaglobulin, precipitates. After centrifugation, the 125I-activity in the precipitate is measured.
*Two-step assay procedure: 3h at RT + 30 min at RT + 15 min centrifugation.