Cisbio Bioassays offers an accurate immunoradiometric assay for thyroxine binding protein (TBG). The assay is based on the competition type principle. The short 30 minute incubation enables results to be obtained very quickly. Added to the dosage of T4, the TBG assay enables the calculation of the T4/TBG ratio, key to a differential diagnosis between hypothyroidism, euthyroidism and hyperthyroidism.
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Thyroxine Binding Globulin (TBG) radioimmunological assay kit
Features
- Working range: 0 ? 80 ?g/mL
- Sample: serum or plasma ? 20 ?L
Applications
- Assessment of the thyroid function
- T4/TBG ratio: differential diagnosis for euthyroid, hypothyroid, hyperthyroid metabolic status
- TBG alone: diagnosis of congenital TBG anomalies
Assay principle
RIP
PEG
The serum TBG competes with the 125I-TBG tracer for a limited number of binding sites on highly specific anti-TBG antibodies. Free TBG is separated from TBG-antibody complex by precipitating the latter with polyethylene glycol (PEG). The 125I-activity in the precipitate is measured. Two-step assay procedure: 30 min at RT + 20 min centrifugation.