Cisbio Bioassays offers an accurate enzymatic (ELISA) assay for measuring the thyroglobulin autoantibodies. It is based on the sandwich type principle and carried out in human serum.
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- TgAb ELISA
Thyroglobulin autoantibody (TgAb) ELISA kit version 2
Features
- Working range: 65 ? 5000 U/mL (NIBSC 65/093)
- Sample: serum ? 50 ?L
- Analytical sensitivity: 9.8 U/mL
Applications
- Autoimmune destruction of the thyroid is associated with the formation of autoantibodies to thyroglobulin
- Diagnosis of autoimmune thyroid diseases
Assay principle
ELISA
Microplate
Tg molecules are coated onto the ELISA plate wells. Protein A-Alkaline Phosphatase conjugate constitutes the liquid phase, which binds to the TgAb from the sample bound to the immobilized Tg. Addition of p-nitrophenyl phosphate (pNPP) enables determination of the Protein a-Alkaline phosphatase conjugate bound to the plate. Assay procedure: 15 min at RT / shaking + 15 min at RT / shaking + 15 min at RT.