Thyroid Peroxidase Autoantibody (TPO-AB) ELISA kit Cisbio Bioassays offers an accurate enzymatic (ELISA) assay for measuring the Thyroid Peroxidas eautoantibodies (TPO-AB). It is based on the sandwich type principle and carried out in human serum.
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Thyroid Peroxidase Autoantibody (TPO-AB) ELISA kit
Features
- Working range: 10 ? 5000 U/mL (WHO 66/387)
- Sample: serum ? 50 ?L
- Analytical sensitivity: 1.05 U/mL
Applications
- Diagnostic value in autoimmune thyroid disease
- Autoimmune destruction of the thyroid is associated with the formation of autoantibodies to thyroid peroxidase
Assay principle
ELISA
Microplate
TPO molecules are coated onto the ELISA plate wells. Protein A-Alkaline Phosphatase conjugate constitutes the liquid phase which binds to the TPOAb from the sample bound to the immobilized TPO. The addition of p-nitrophenyl phosphate (pNPP) enables determination of the Protein a-Alkaline phosphatase conjugate bound to the plate. Assay procedure: 15 min at RT / shaking + 15 min at RT / shaking + 15 min at RT in the dark.