Cisbio Bioassays offers an accurate immunoradiometric assay for TSH, it is based on the sandwich type principle. The high sensitivity and reliability of the assay enable classification of patients for hypothyroidism, euthyroidism and hyperthyroidis
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Human thyrotropin (hTSH) radioimmunometric assay kit
Features
- Working range: 0 ? 65 ?IU/mL (2nd IRP 80/558)
- Sample: serum or plasma ? 200 ?L
- Analytical sensitivity: 0.03 ?IU/mL
Applications
- First serum parameter to evaluate the thyroid function
- Evaluation of TSH following TRH test to discriminate for euthyroidism, hyperthyroidism or hypothyroidism
Assay principle
IRMA
Coated tubes
Two monoclonal anti-hTSH antibodies are used in the sandwich. The first monoclonal antibody is adsorbed on to the tube walls. The second monoclonal antibody, radiolabeled with iodine-125 is used as the tracer.
*One-step assay procedure: 2h at RT / shaking.