The only patented * assay targeting the core of the molecule (145-245), Cisbio’s Chromoa R assay offers exceptional sensitivity, specificity, and reproducibility by using two monoclonal antibodies to measure intact and fragmented Chromogranin A (CgA).This enhances the reliability of results. Chromogranin A measurements in neuroendocrine tumors are established in the guidelines** as an aid for diagnosis, treatment efficiency and prognostic factors. Developed under a sandwich-type ELISA format, Chromoa R is simple to use and can be easily adapted to automated systems for implementation in laboratories of all sizes. *EP1078266,US 6,632,624 ** Guidelines
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Chromogranin A ELISA kit
Features
- Working range: 7 ? 820 ng/mL
- Sample: serum or plasma ? 20 ?L
- Analytical sensitivity: 7 ng/mL
- Functional sensitivity: 11 ng/mL
Applications
- High specificity and sensitivity in tumors of neuroendocrine origin
- Diagnosis and follow-up in pheochromocytoma, gastrinoma, carcinoid tumors
- Adverse prognostic factor in prostate cancer
Assay principle
ELISA
Microplate
Two monoclonal antibodies directed against the unprocessed central domain are used for the sandwich. A first monoclonal antibody is immobilized on the microplate, while a second monoclonal antibody is conjugated to Horse-Radish-Peroxidase.
*Two alternative procedures: long protocol (16h at RT + 2h at RT / shaking + 10 min at RT / shaking) or short protocol (2h at RT / shaking + 2h at RT / shaking + 10 min at RT / shaking).