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Free Prostate Specific Antigen (FPSA) immunoradiometric assay kit

Through our know-how and expertise gained over 30 years in the tumor marker field, Cisbio Bioassays offers an accurate immunoradiometric assay for Free Prostate Specific Antigen. The FPSA-RIACT assay is based on a sandwich-type assay performed on serum or plasma, and is available in a 50 tube format for small runs.

Features

  • Working range: 0.02 ? 20 ng/mL (ng CIS = 0.83 y ng/mL 1st IS 96/668 or 10 ng/mL CIS = 12 ng/mL 1st IS 96/668)
  • Sample: serum or EDTA, heparin plasma ? 100 ?L
  • Analytical sensitivity: 0.02 ng/mL
  • Functional sensitivity: 0.16 ng/mL

Applications

  • Free PSA measurement is combined with total PSA for calculation of the Free PSA / Total PSA percentage
  • Ratio higher in cases of benign prostatic hypertrophy than in those of prostate cancer
  • Free PSA / Total PSA ratio is a valuable tool for the differential diagnosis of prostate cancer
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Ref: FPSA-RIACT

Assay principle

IRMA

Coated tubes

Two anti-PSA monoclonal antibodies are used for the sandwich. The first monoclonal antibody is adsorbed onto the tube walls, while the second monoclonal antibody specific to free PSA and radiolabelled with iodine-125 is used as the tracer.
*One-step assay procedure: 18h at RT.

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