The HGH-RIACT kit has a one step protocol. The use of two monoclonal antibodies, one labelled with iodine 125 and the other adsorbed onto the inner tube walls, enables a specific, sensitive assay of the 22 kDa form of the hormone (detection limit 0.01 ng /mL). In conformity with European recommendations, the kit has been standardized with the 2nd IS 98/574 (1ng=3?IU).
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Human growth hormone (hGH) immunoradiometric assay kit
Features
- Working range: 0.01 ? 25.2 ?IU/mL (2nd IS 98/574) [1 ng = 3 ?IU]
- Sample: serum ? 50 ?L
- Analytical sensitivity: 0.01 ?IU/mL
Applications
- Exploration of growth disorders
- Monitoring of treatment by recombinant hGH
- Follow-up of acromegaly treatment
Assay principle
IRMA
Coated tubes
Two monoclonal antibodies specific to 22kD hGH are used for the sandwich. The first monoclonal antibody is adsorbed onto the tube walls. The second monoclonal antibody, radiolabelled with iodine-125, is used as the tracer.
*One-step assay procedure: 2h at RT / shaking.