This methodology enables a tedious extraction step to be avoided, while ensuring that binding proteins do not interfere with the assay. Furthermore, the use of a highly specific monoclonal couple gives the assay a high level of specificity.
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Somatomedin C (IGF-I) immunoradiometric assay kit
Features
- Working range: 1 ? 1000 ng/mL (IRS 87/518)
- Sample: serum or plasma ? 25 ?L - predilution required (1/31)
- Analytical sensitivity: 1 ng/mL
Applications
- Diagnosis and treatment monitoring for growth-related disorders in children
- Adult hGH deficiency assessment
- Follow-up of acromegaly treatment
- Nutritional state assessment
Assay principle
IRMA
Coated tubes
Two monoclonal antibodies directed against two different antigenic sites of the IGF-I molecule are used for the sandwich. The first monoclonal antibody is adsorbed onto the tube walls. The second monoclonal antibody, radiolabelled with iodine-125, is used as the tracer. A predilution of the samples (1/31) with an acidic solution is required to strip the carrier protein (BP). This carrier protein is then saturated by IGF-II. The use of IGF-II molecules in the assay avoids any reassociation between IGF-I and the carrier protein (IGF-BP).
*Two-step assay procedure: 2h at RT / shaking + 2h at RT / shaking.