Cisbio Bioassays offers an accurate enzymatic (ELISA) assay for measuring the Acetylcholine Receptor (AChRAb) autoantibodies. It is based on the competition type principle and carried out in human serum.
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Acetylcholine Receptor Autoantibody (AChRAb) ELISA kit
Features
- Working range: 0.5 ? 20 nmol/L
- Sample: serum ? 100 ?L
- Analytical sensitivity: 0.25 nmol/L
Applications
- Contribution to the diagnosis of a failure of the neuromuscular junction in myasthenia gravis
- Follow-up of myasthenia gravis
Assay principle
ELISA
Microplaque
The principle of the assay is based on competition between AChRAb in human serum and MAb1 coated on the microplate, and/or MAb2 and/or MAb3 which are labeled with biotin for binding to the Acetylcholine Receptor (AChR). The bound MAb2 and MAb3-biotin are detected by the addition of Streptavidin Peroxidase (SA-POD). The unbound SA-POD in excess is washed off before the addition of the peroxidase substrate TMB. Assay procedure: 16h at 2-8°C + 1 h at RT / shaking + 1 h at RT / shaking + 30 min at RT / Shaking + 30 min at RT.