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  • AChRAb RIA

Acetylcholine Receptor Autoantibody (AChRAb) radioreceptor assay kit

Cisbio Bioassays offers an accurate immunoradiometric (RIA) assay for measuring the anti-Acetylcholine Receptor autoantibodies (AChRAb). It is based on the sandwich type principle and carried out in human serum and EDTA plasma.

Features

  • Working range: negative and positive controls
  • Sample: serum or EDTA plasma ? 5 ?L
  • Analytical sensitivity: 0.02 nmol/L

Applications

  • Contribution to diagnosis of the failure of neuromuscular junction in myasthenia gravis
  • Follow-up of myasthenia gravis
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Ref: ACHR-AB

Assay principle

Radioreceptor assay RRA

Centrifugation

The receptors radiolabelled with iodine-125 are incubated with test sera and any resulting complexes of labeled receptor and receptor antibody are immunoprecipitated with anti-human immunoglobulin. After centrifugation and washing, the precipitate is counted. Assay procedure: 2h at RT + 2h or 18h at 2-8°C + centrifugation 20 min + centrifugation 20 min.

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