Cisbio Bioassays offers an accurate enzymatic (ELISA) assay for measuring the Aquaporin-4 (AQP4) autoantibodies. It is based on the sandwich type principle and carried out in human serum.
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- AQP4Ab ELISA
Aquaporin-4 (AQP4) autoantibody (AQP4Ab) ELISA kit version 2
Features
- Working range: 1.5 ? 80 U/mL
- Sample: serum ? 50 ?L
- Analytical sensitivity: 0.17 U/mL
Applications
- Diagnosis of neuromyelitis optica (NMO) (Devic’s syndrome) or neuromyelitis optica spectrum disorder (NMOSD)
- Distinguishing NMOSD from multiple sclerosis early in the course of the disease
Assay principle
ELISA
Microplaque
AQP4 molecules are coated onto the ELISA plate wells. AQP4-biotin complexes constitute the liquid phase. AQP4 Ab from the sample bound to the AQP4 coated on the well will also interact with AQP4-biotin via an AQP4 Ab bridge. Addition of streptavidin peroxidase (SA-POD) detects the bound AQP4-biotin. The unbound SA-POD in excess is washed off before addition of the peroxidase substrate, TMB. Assay procedure: 2h at RT / shaking + 20 min at RT / shaking + 20 min at RT.