Cisbio Bioassays offers an accurate enzymatic (ELISA) assay for measuring the Tyrosine phosphatase Islet Antigen autoantibodies (IA-2Ab). It is based on the sandwich type principle and carried out in human serum.
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- IA2Ab ELISA
Tyrosine phosphatase Islet Antigen (IA-2) autoantibody (IA2Ab) ELISA kit version 2
Features
- Working range: 7.5 ? 4000 U/mL (NIBSC 97/550)
- Sample: serum ? 50 ?L
- Analytical sensitivity: 1.25 U/mL
Applications
- IA2 autoantibodies are strongly associated with type I diabetes mellitus (insulin-dependent diabetes mellitus - IDDM)
Assay principle
ELISA
Microplaque
IA2 molecules are coated onto the ELISA plate wells. IA2-biotin complexes constitute the liquid phase. IA2-Ab bound to the IA2 coated onto the well will also interact with IA2-biotin via an IA2-Ab bridge. Addition of streptavidin peroxidase (SA-POD) detects the bound IA2-biotin. The unbound SA-POD in excess is washed off before the addition of the peroxidase substrate, TMB. Assay procedure: 16h at 2-8°C + 1h at 2-8°C + 20 min at RT / shaking + 20 min at RT.