Cisbio offers a accurate immunoradiometric assay for measuring the P-Type Voltage-Gated Calcium Channel (VGCC) autoantibodies. It is carried out in human serum.
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P-Type Voltage-Gated Calcium Channel (VGCC) Autoantibody RIA kit assay
Features
- Measurement range: 1 negative control et 1 positive control
- Sample: serum: 25 ?L
- Analytical limit detection: 2,86 pmol/L
Applications
- P-Type Voltage-Gated Calcium Channel (VGCC) channelopathies
- Neurological disorders such as Lambert-Eaton myasthenic syndrome (LEMS)
Assay principle
RIP
R?actif immunoprecipitant
The sample containing VGCC Ab is incubated with the 125I VGCC tracer. After addition of antihuman IgG and centrifugation, the precipitate is counted.
*Assay procedure: 1h RT + 1h RT + 20 min RT (centrifugation).