Cisbio Bioassays offers an accurate enzymatic (ELISA) assay for measuring the Glutamic Acid Decarboxylase autoantibodies (GAD-Ab). It is based on the sandwich type principle and carried out in human serum.
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Glutamic Acid Decarboxylase autoantibody (GAD-Ab) ELISA kit
Features
- Working range: 5 ? 2000 U/mL (NIBSC 97/550)
- Sample: serum ? 25 ?L
- Analytical sensitivity: 0.57 U/mL
Applications
- GAD65 autoantibodies are important serological markers of type 1 diabetes mellitus
Assay principle
ELISA
Microplaque
GAD65 molecules are coated onto the ELISA plate wells. GAD65-biotin complexes constitute the liquid phase. GAD-Ab from the samples bind to the GAD65 immobilized onto the well will also interact with GAD65-biotin via a GAD-Ab bridge. Addition of streptavidin peroxidase detects the bound GAD65-biotin. The unbound streptavidin peroxidase in excess is washed off before the addition of the peroxidase substrate, TMB. Assay procedure: 1h at RT / shaking + 1h at RT / shaking + 20 min at RT / shaking + 20 min at RT in the dark.