Cisbio Bioassays offers an accurate enzymatic (ELISA) assay for measuring the Glutamic acid decarboxylase (GAD) and IA-2 autoantibodies. It is based on the sandwich type principle and carried out in human serum.
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- GADAb & IA-2Ab ELISA
Two screen islet cell autoantibody (GADAb & IA-2Ab) ELISA kit
Features
- Working range: 4 ? 450 U/mL (NIBSC 97/550)
- Sample: serum ? 50 ?L
- Analytical sensitivity: 0.17 U/mL
Applications
- Simultaneous measurement of GAD and IA-2 autoantibodies in the same sample
- Important serological markers in type 1 diabetes mellitus
Assay principle
ELISA
Microplaque
GAD65 and IA-2 molecules are coated onto the ELISA plate wells. GAD65-biotin and IA-2-biotin complexes constitute the liquid phase. GAD-Ab or IA-2-Ab from the sample bind to the GAD65 and to IA-2 immobilized onto the well and interact with GAD65-biotin and IA-2 biotin via a GAD-Ab or IA-2-Ab bridge. Addition of streptavidin peroxidase detects the bound GAD65-biotin and IA-2 biotin. The unbound streptavidin peroxidase in excess is washed off before the addition of the peroxidase substrate TMB. Assay procedure: 16h at 2-8°C + 1h at RT / shaking + 20 min at RT / shaking + 20 min at RT.