The ZNT8AB ELISA kit is used for the quantitative determination of ZNT8 autoantibodies in human serum. Cisbio Bioassays offers an accurate enzymatic (ELISA) assay for measuring the Zinc transporter 8 (ZnT8) autoantibodies. It is based on the sandwich type principle and carried out in human serum.
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Zinc Transporter 8 (ZnT8) autoantibody (ZnT8Ab) ELISA kit
Features
- Working range: 10 ? 2000 U/mL
- Sample: serum ? 25 ?L
- Analytical sensitivity: 1.2 U/mL
Applications
- Quantification of ZnT8 autoantibodies specific to R325 or to W 325 or to residue 325 non specific variants
- Important serological markers in type 1 diabetes mellitus
Assay principle
ELISA
Microplaque
ZnT8 molecules are coated onto the ELISA plate wells. ZnT8-biotin complexes constitute the liquid phase. ZnT8-Ab from the sample bound to the ZnT8 coated onto the well will also interact with ZnT8-biotin via a ZnT8-Ab bridge. Addition of streptavidin peroxidase (SA-POD) detects the bound ZnT8-biotin. The unbound SA-POD in excess is washed off before the addition of the peroxidase substrate, TMB. Assay procedure: Overnight (16h-20h) at 2-8°C + 1 h at 2-8°C + 20 min. RT / shaking + 20 min. TA in the dark.