The REN-CT2 kit is based on a competition principle. After generation of Angiotensin 1, the assay procedure is a one step protocol. Measurement of plasma renin activity (PRA) in hypertensives is an important aid in the differential diagnosis of primary and secondary aldosteronism.
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- Angiotensin I RIA
Angiotensin I radioimmunoassay kit
Features
- Working range: 0 ? 25 ng/mL (MRC 71/328)
- Sample: EDTA plasma; 50 ?L
- Analytical sensitivity: 0.15 ng/mL
Applications
- Evaluation of the renin-angiotensin system
- Angiotensin I level represents the Plasma Renin Activity (PRA)
- Assessment and follow-up of hypertensive patients
- Differential diagnosis between primary and secondary aldosteronism
Assay principle
RIA
Coated tubes
Prior to the assay, preparation of the samples is required for generation of angiotensin I in the plasma samples. The principle of the assay is based on a competition between angiotensin I radiolabelled with iodine-125 and angiotensin I contained in the samples for a fixed and limited number of anti-angiotensin I polyclonal antibody binding sites adsorbed onto the tube walls.
*Assay procedure: 1- Preparation of the plasma samples: blanks (ice bath) and generated samples (90 min at 37°C then ice bath) 2- Assay protocol 90 min at RT / shaking.