ELSA-ACTH is a solid phase sandwich-type assay using two monoclonal antibodies, thus enabling a reliable quantitative determination of the whole corticotropic hormone. Because of the specificity of the solid phase, there is no interference with peptide 1-24 synacthene in the standard concentrations. With a detection limit of 2 pg/mL, the assay offers very good sensitivity.
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- ACTH RIA
Whole corticotropic hormone (ACTH) immunoradiometric assay kit
Features
- Working range: 2 ? 2000 pg/mL
- Sample: EDTA plasma ? 200 ?L
- Analytical sensitivity: 2 pg/mL
Applications
- Exploration of cortico-adrenal, congenital adrenal hyperplasia and glucocorticoid hypercorticism
- Evaluation of ACTH secretion following stimulation or suppression tests to assess hypothalamo-hypophyso-adrenal disturbances
- Etiological diagnosis of Cushing’s syndromes: ACTH dependent or ACTH independent
- Etiological diagnosis of adrenal deficiencies: primary adrenal deficiency - Addison’s disease; secondary adrenal deficiencies ? panhypopituitarism, weaning off cortico-therapy
Assay principle
IRMA
Solid phase ELSA system
Two monoclonal anti-ACTH molecule antibodies are used for the sandwich. The first monoclonal antibody, specific to the N-terminal part of ACTH, is coated on the ELSA solid phase, while the second monoclonal antibody, which is specific to the C-terminal part of ACTH and is radiolabelled with iodine-125, is used as the tracer.
*One-step assay procedure: 20h at RT.