Based on the RIA principle of a competition assay, R-GM-100 is carried out in a single one-hour step. The kit demonstrates excellent analytical performances through a very good sensitivity (analytical sensitivity 0.05 ng/mL) as well as very high specificity as concerns other androgens.
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- Androstenedione RIA
Androstenedione radioimmunoassay kit
Features
- Working range: 0.05 ? 10 ng/mL
- Sample: serum ? 25 ?L
- Analytical sensitivity: 0.05 ng/mL
Applications
- Virilizing congenital adrenal hyperplasia
- Adrenal and ovarian virilizing tumors
- Cushing syndrome
- Polycystic ovary syndrome
- Hirsutism in women
- Ovarian stromal hyperthecosis
- 3β-hydroxysteroid dehydrogenase deficiency
Assay principle
RIA
Coated tubes
The principle of the assay is based on a competition between androstenedione from the sample and 125I-androstenedione for a fixed and limited number of anti-androstenedione polyclonal antibody binding sites coated onto the tube wall.
*One-step assay procedure: 1h at 37°C.