ESTR-CTRIA is based on the competition principle for binding with an immunoserum adsorbed onto the inner walls of the coated tubes. The 2h30min incubation step is compatible with a daily follow-up of assays, if necessary. Its range is as high as 18 000 pmol/L, enabling an accurate assay without dilution, even for the high values observed in ovulation induction.
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- Estradiol RIA
Human serum or plasma estradiol (E2) radioimmunoassay kit
Features
- Working range: 0 ? 5000 pg/mL (GCMS) [Estradiol in pmol/L] = [Estradiol in pg/mL] x 3.67
- Sample: serum or plasma ? 100 ?L
- Analytical sensitivity: 4 pg/mL
- Functional sensitivity: 8 pg/mL
Applications
- Assessment of ovarian function in females
- Assessment during the use of clomiphene or hMG for diagnostic purposes (exploration of amenorrhea or anovulation)
- Assessment for therapeutic purposes (occurrence of pregnancy)
- Monitoring of ovulation and in-vitro fertilization
- Evaluation of feminization, including gynecomastia, in males
Assay principle
RIA
Coated tubes
The principle of the assay is based on competition between 125iodine radiolabelled estradiol and estradiol contained in the samples for a fixed and limited number of anti-estradiol polyclonal antibody binding sites adsorbed onto the tube walls.
*Assay procedure has two options: 1- female sera under ovulation induction 1h30 at RT / shaking; 2- other applications 2h30 at RT / shaking.