This assay is very useful for the early diagnosis of congenital hyperadrenalism (21-hydroxylasis deficiency).The 17-OHP-NN kit has been developed for use on dried blood spots. It is used during screening for hyperadrenalism in new-borns, during the first few days after birth.
- Home
- Products
- In-Vitro Diagnostics
- Endocrinology other
- Neonatal 17OHP RIA
17 alpha-hydroxy-progesterone (17α-OHP) in blood spots from newborns - RIA kit
Features
- Working range: 0 ? 600 nmol/L
- Sample: dried blood spot on paper disks
- Analytical sensitivity: 1 nmol/L
Applications
- Neo-natal congenital adrenal hyperplasia screening
- Early diagnosis of congenital adrenal hyperplasia
Assay principle
Double antibody radioimmunoassay RIP
Immunoprecipitating reagent
The principle of the assay is based on competition between 17-OHP radiolabelled with iodine-125 and 17-OHP contained in the sample to be assayed for a given number of anti-17-OHP polyclonal antibody sites. The separation of the free and bound fractions uses an immunoprecipitating reagent in which a second antibody has been preprecipitated in excess.
*Three-step assay procedure: 30 min at RT + 1h at 37°C / shaking + 30 min at 37°C / shaking.