PROGEST-RIA's sensitivity (detection limit 0.05 ng/mL) make it a well-adapted tool for luteal deficiency assays (sterility, repeated spontaneous miscarriages). The assay is based on a one step protocol.
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- Progesterone RIA
Progesterone radioimmunoassay kit
Features
- Working range: 0 ? 36 ng/mL [Progesterone in nmol/L = 3.18 X Progesterone in ng/mL]
- Sample: serum ? 50 ?L
- Analytical sensitivity: 0.05 ng/mL
Applications
- Ascertaining whether ovulation occurred in a menstrual cycle
- Evaluation of patients with sterility due to luteal phase defects
Assay principle
RIA
Coated tubes
The principle of the assay is based on competition between 125I radiolabelled progesterone and progesterone contained in the samples for a fixed and limited number of anti-progesterone polyclonal antibody binding sites, adsorbed onto the tube walls.
*One-step assay procedure: 2h at 37°C.