The TESTO-CT2 assay is based on a one step protocol. The detection limit, as low as 0.1 nmol/L, suits endocrinology requirements (for children < 0.35 nmol /L and for women 2.4 nmol /L). An optional extraction protocol is available to enhance technical performance when assaying female sera. As recommended, the kit is standardized against GC-MS (working range to 50 nmol/l).
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- Testosterone RIA
Testosterone radioimmunoassay kit
Features
- Working range: 0.1 ? 50 nmol/L
- Sample: serum ? 25 ?L
- Analytical sensitivity: 0.1 nmol/L
- Functional sensitivity: 0.3 nmol/L
Applications
- Androgen deficiency in men
- Delayed or precocious puberty in young men
- Androgen excess in women: hirsutism, virilism
- Polycystic ovary syndrome
- Endocrine therapy monitoring
Assay principle
RIA
Coated tubes
The principle of the assay is based on competition between 125I radiolabelled testosterone and testosterone contained in the samples for a fixed and limited number of anti-testosterone polyclonal antibody binding sites adsorbed onto the tube walls.
*One-step assay procedure: 1h at 37°C..