The kit's principle is based on a competition between the endogenous gastrin and gastrin labeled with iodine 125 for a limited quantity of anti-serum binding sites. The standard range stretches from 0-1500 ?U/mL and the incubation time is 2h15 mn at RT.
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- Gastrin RIA
Human Gastrin radioimmunoassay kit
Features
- Working range: 0 ? 1500 ?U/mL (1 ?U MRC 68/439 = 1.05 pg MRC 68/439)
- Sample: serum or plasma ? 100 ?L
- Analytical sensitivity: 10 ?U/mL
Applications
- Early diagnosis of Zollinger-Ellison syndrome
- Diagnosis of gastrinoma with knowledge of patient’s basal and secretin-stimulated serum gastrin measurement
- Investigation of patients with antral G cell hyperplasia, vagotomy, atrophic gastritis
- Investigation of patients with Biermer’s anemia
Assay principle
RIP
Immunoprecipitating reagent
In the first step, gastrin contained in the samples and 125I-radiolabelled gastrin compete for a given limited number of anti-gastrin antibody sites. In the second step, the gastrin or 125I-radiolabelled gastrin antibody complex reacts with an anti-rabbit gammaglobulin and precipitates. The 125I-activity in the precipitate is measured.
*Three-step assay procedure: 2h at RT + 15 min at RT + 15 min centrifugation.