The HYAL-US kit offers a very good analytical sensitivity (2.7 ng/mL) and an extended dynamic range which enables the number of pre-dilution steps to be limited. The ELISA format can be easily adapted to automated systems for implementation in laboratories of all sizes. This kit is "For Research Use Only, not for use in diagnostic procedures".
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- Hyaluronic acid ELISA (RUO)
Hyaluronic acid ELISA kit (RUO)
Features
- Working range: 2.7 ? 1000 ng/mL
- Sample: serum or EDTA plasma ? 100 ?L
- Analytical sensitivity: 2.7 ng/mL
Applications
작용원리
ELISA
Microplate
히알루론산 결합 단백질은 샌드위치법에 사용된다. 히알루론산 결합 단백질은 마이크로테이터 플레이트에 코팅되어 있다. 히알루론산 결합 단백질과 결합된 Horse-radish peroxidase이 검출된다.
* step 검사 절차: RT(실온)에서 시간 / Shaking + RT(실온)에서 30분 / Shaking + RT(실온)에서 30분 / Shaking.
Assay procedure: 2h at RT / shaking + 30 min at RT / shaking + 30 min at RT / shaking.