Though other methods exist, the radioimmunoassay determination of immunoreactive trypsin in neonatal blood spots has been found highly suitable for the diagnosis of Mucoviscidosis. As described below, it is particularly well-adapted to neonatal screening. Neonatal blood spots are collected on filter paper discs.
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- Neonatal Trypsin RIA
Human trypsin (htrypsin) in blood spots of newborns - radioimmunoassay kit
Features
- Working range: 0 ? 120 ng/mL
- Sample: dried blood spot on paper disks
- Analytical sensitivity: 4.0 ng/mL
Applications
- Neonatal screening of cystic fibrosis
- Early postnatal diagnosis of cystic fibrosis
- Molecular biology being the decisive tool for diagnosis of the disease.
작용원리
RIP
Immunoprecipitating reagent
첫 번째 단계에서는 trypsin과 Iodine 125가 표지된 trypsin이 매우 특정한 anti-trypsin 항체를 위해 경쟁한다. 두 번째 단계에서는 trypsin이나 Iodine 125가 표지된 trypsin 항체 결합물이 anti-rabbit gammaglobulin과 침전물과 반응한다. 침전물의 Iodine 125 방사능량을 측정한다.
*검사 절차: RT(실온)에서 직사광선을 피해 17시간 + RT(실온)에서 직사광선을 피해 2시간 + RT(실온)에서 직사광선을 피해 30분 + 2-8°C에서 20분 원심분리.