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Procollagen-III-peptide (PIIINP) ELISA kit

Procollagen-III-peptide ELISA assay The P3NP-ELISA test enables the determination of human procollagen III peptide. It is based on the principle of a very easy to use sandwich assay. The 30 ?l blood sample is convenient for patients. The transformation of functioning liver tissue into connective tissue is detectable through a raised serum procollagen III peptide level. This is the case, for instance, in alcoholic or virus induced forms of liver fibrosis. The parameter is suitable for monitoring the course of liver disorders and particularly in psoriatic patients treated by Methotrexate. The procollagen III peptide determination enables to reduce the number of biopsy for a better patient comfort and cost savings. Procollagen III N-terminal Propeptide (P3NP) is synthetized in fibroblasts and released from the precursor peptide during the synthesis and deposition of Type III Collagen. Related product: HYAL-ELISA ( Hyaluronic acid ELISA assay)

Features

  • Working range: 0.036 ? 30 ?g/L
  • Sample: serum or plasma ? 30 ?L
  • Analytical sensitivity: 0.036 ?g/L
  • Functional sensitivity (12.5%): 2.2 ?g/L

Applications

  • Clinical interest
  • - High P3NP concentrations in the following indications:
  • > fibrosis and cirrhosis in the non alcooholic origin
  • >lung fibrosis, chronic polyarthritis, collagenose, myocardal infection, acromegaly amd multiple data
  • - Follow-up of the disease
  • - Correlation between P3NP and histological data in fibrosis and cirhhosis
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Ref: P3NP-ELISA

작용원리

P3NP-ELISA 검사는 비색법의 ELISA 타입의 면역측정이며, 샌드위치법에 사용된다. 마이크로 플레이트에 고정된 첫 번째 단일클론성 항체는 칼리브레이터와 샘플에 포함된 PIIINP 단백질을 포착한다. HRP와 결합된 두 번째 단일클론성 항체는 결합된 단백질을 인식한다. 결합되지 않은 시약은 제거한 후, 색도 반응은 과산화 효소기질인 TMB를 더하여 시작된다. 이 반응은 산성물질을 첨가하여 멈추고, 각 웰의 광밀도(OD)를 450 nm에서 측정한다.

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