Through our know-how and our expertise gained over 30 years in the tumor marker field, Cisbio Bioassays offers an accurate immunoradiometric assay for antigen CA 125II. The ELSA-CA125II kit is based on a sandwich assay, using reference antibodies (OC 125).
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- CA125 II RIA
CA 125 antigen (CA125 II) immunoradiometric assay kit
Features
- Working range: 0 - 500 U/mL
- Sample: serum or non-citrated plasma ? 100 ?L
- Analytical sensitivity: 0.5 U/mL
Applications
- Differential diagnosis of ovarian tumors
- Evaluation of initial treatment efficacy
- Early detection of relapse or metastasis
- Decision to undertake second-look surgery
- Long-term follow-up of patients in remission
작용원리
IRMA
Solid phase ELSA system
두개의 단일클론성 anti-CA 125 항체는 샌드위치법에 사용된다.
첫번째 단일클론성 항체인 anti CA 125 II 는 ELSA 고체상에 흡착되어 있고, Iodine 125가 표지된 두번째 단일클론성 항체인 anti-CA 125는 추적자로 사용된다.
*1 step 검사 절차: RT(실온)에서 18-20시간.