상정인터내셔널(주) SANG CHUNG INTERNATIONAL Co.,Ltd.

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Free β subunit of human chorionic gonadotropin (FβHCG) immunoradiometric assay kit

The use of monoclonal antibodies directed against epitopes only present on the free bHCG subunit enables a specific and ultrasensitive assay of this subunit. Furthermore, the assay presents no cross-reactions with LH, TSH, FSH or native HCG. The ELSA-FbHCG assay is particularly wel-adapted to the diagnosis and monitoring of trophoblastic tumors, either of placental (moles, chorionic carcinoma, chorioepithelioma) or testicular origin (seminomatous germ cell tumors or non-seminomatous tumors having a trophoblastic cell component). The use of such an ultrasensitive method means early diagnosis of therapeutic escape and of metastatic spread.

Features

  • Working range: 0.04 ? 50 ng/mL (1 ng CIS = 1 mIU ; IRP 75/551)
  • Sample: serum or EDTA plasma ? 100 ?L
  • Analytical sensitivity: 0.04 ng/mL

Applications

  • Tumor marker in trophoblastic tumors of placental origin ? moles, chorionic carcinoma, chorioepithelioma
  • Tumor marker in trophoblastic tumors of testicular origin ? seminomatous germ cell tumors or non seminomatous tumors having a trophoblastic component
  • Diagnosis of these trophoblastic tumors
  • Surveillance of these trophoblastic tumors
  • Early detection of therapeutic escape and of metastatic spread
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Ref: ELSA-FBHCG

작용원리

IRMA

Solid phase ELSA system

두개의 단일클론성 항체는 샌드위치법에 사용된다.
첫번째 단일클론성 항체는 ELSA 고체상에 흡착되어 있다. Iodine 125가 표지된 두번째 단일클론성 항체는 추적자로 사용된다.
*1 step 검사 절차: RT(실온)에서 1시간 / Shaking + RT(실온)에서 2시간 / Shaking.

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