Through our know-how and expertise gained over 30 years in the tumor marker field, Cisbio Bioassays offers an accurate immunoradiometric assay for Neuron Specific Enolase. The ELSA-NSE kit is based on a sandwich-type assay and performed on serum.
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Neuron Specific Enolase (NSE) immunoradiometric assay kit
Features
- Working range: 0 - 200 ng/mL
- Sample: serum only - 50 ?L
- Analytical sensitivity: 0.3 ng/mL
Applications
- Tumor marker in tumors of neuroectodermic or neuroendocrine origin
- Evaluation of prognosis in small-cell lung cancer and follow-up of cytotoxic treatment
- Differential diagnosis between Wilms tumor and neuroblastoma in presence of abdominal mass syndrome
- Prognostic factor in neuroblastoma
- Surveillance of neuroblastoma
작용원리
IRMA
Solid phase ELSA system
두개의 단일클론성 항체는 샌드위치법에 사용된다.
첫번째 단일클론성 항체 anti-NSE는 ELSA 고체상에 흡착되어 있고, Iodine 125가 표지된 두번째 단일클론성 항체 anti-NSE 는 추적자로 사용된다.
*1 step 검사 절차: RT(실온)에서 3시간 / Shaking.