The BI-INS-IRMA kit is an immunoradiometric assay (IRMA) used on serum or on EDTA or heparined plasma samples. Two monoclonal antibodies, one radiolabelled with iodine 125 and the other adsorbed onto the inner walls of the tube, enable a sensitive (detection limit 0.2 ?UI/mL) specific assay of human insulin, in particular because of a cross-reaction <0.0001 with human pro insulin.
- Home
- 제품소개
- In-Vitro Diagnostics
- Diabetes
- Free and total Insulin RIA
Insulin immunoradiometric assay kit
Features
- Working range: 0 ? 500 ?IU/mL or 0 ? 20 ng/mL (1st IRP 66/304)
- Sample: serum or plasma ? 50 ?L
- Analytical sensitivity: 0.2 ?IU/mL (8 pg/mL)
- Functional sensitivity: -
Applications
- Diagnosis of diabetes mellitus
- Early detection of diabetes mellitus before clinical signs
- Monitoring of metabolic control in diabetic patients
- Prognosis factor for the risk of coronary incident
- Diagnosis of insulinoma
작용원리
IRMA
Coated tubes
두개의 단일클론성 anti-insulin 항체는 샌드위치법에 사용된다.
첫번째 단일클론성 항체는 튜브 벽에 흡착되어 있고, Iodine 125가 표지된 두번째 단일클론성 항체는 추적자로 사용된다.
*두가지 검사 절차: 1- 면역반응성 insulin(free insulin과 anti-insulin 항체와 결합된 insulin)의 측정: RT(실온)에서 2시간 / shaking; 2- free insulin의 측정: 15분 원심분리 + RT(실온)에서 2시간 / shaking.