ELSA-ACTH is a solid phase sandwich-type assay using two monoclonal antibodies, thus enabling a reliable quantitative determination of the whole corticotropic hormone. Because of the specificity of the solid phase, there is no interference with peptide 1-24 synacthene in the standard concentrations. With a detection limit of 2 pg/mL, the assay offers very good sensitivity.
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- ACTH RIA
Whole corticotropic hormone (ACTH) immunoradiometric assay kit
Features
- Working range: 2 ? 2000 pg/mL
- Sample: EDTA plasma ? 200 ?L
- Analytical sensitivity: 2 pg/mL
Applications
- Exploration of cortico-adrenal, congenital adrenal hyperplasia and glucocorticoid hypercorticism
- Evaluation of ACTH secretion following stimulation or suppression tests to assess hypothalamo-hypophyso-adrenal disturbances
- Etiological diagnosis of Cushing’s syndromes: ACTH dependent or ACTH independent
- Etiological diagnosis of adrenal deficiencies: primary adrenal deficiency - Addison’s disease; secondary adrenal deficiencies ? panhypopituitarism, weaning off cortico-therapy
작용원리
IRMA
Solid phase ELSA system
두개의 단일클론성 anti-ACTH 분자 항체는 샌드위치법에 사용된다.
ACTH의 N-terminal 부분으로 특정되는 첫번째 단일클론성 항체는 ELSA 고체상에 흡착되어 있고, ACTH의 C-terminal 부분으로 특정되는 두번째 단일클론성 항체는 Iodine 125가 표지되어 있으며 추적자로 사용된다.
*1 step 검사 절차: RT(실온)에서 20시간.