ESTR-CTRIA is based on the competition principle for binding with an immunoserum adsorbed onto the inner walls of the coated tubes. The 2h30min incubation step is compatible with a daily follow-up of assays, if necessary. Its range is as high as 18 000 pmol/L, enabling an accurate assay without dilution, even for the high values observed in ovulation induction.
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- In-Vitro Diagnostics
- Endocrinology other
- Estradiol RIA
Human serum or plasma estradiol (E2) radioimmunoassay kit
Features
- Working range: 0 ? 5000 pg/mL (GCMS) [Estradiol in pmol/L] = [Estradiol in pg/mL] x 3.67
- Sample: serum or plasma ? 100 ?L
- Analytical sensitivity: 4 pg/mL
- Functional sensitivity: 8 pg/mL
Applications
- Assessment of ovarian function in females
- Assessment during the use of clomiphene or hMG for diagnostic purposes (exploration of amenorrhea or anovulation)
- Assessment for therapeutic purposes (occurrence of pregnancy)
- Monitoring of ovulation and in-vitro fertilization
- Evaluation of feminization, including gynecomastia, in males
작용원리
RIA
Coated tubes
해당 키트의 원리는 Iodine 125가 표지된 estradiol과 샘플에 있는 estradiol간의 경쟁반응에 기초한다.
튜브에 흡착된 다클론성 anti- estradiol 항체와 반응한 추적자의 결합 방사능량을 측정한다.
*두가지 검사 절차: 1-배란 유도하의 여성혈청: RT(실온)에서 1시간 30분 / Shaking; 2-기타: RT(실온)에서 2시간 30분 / Shaking.