The HYAL-ELISA kit offers an extended dynamic range which enables the number of pre-dilution steps to be limited. Because of the very good analytical sensitivity (2.7 ng/mL), a reliable patient and treatment follow-up can be done. Stratified age and sex normal values are convenient for data interpretation. The Elisa format can be easily adapted to automated systems for implementation in laboratories of all sizes.
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- Hyaluronic acid ELISA (CE)
Hyaluronic acid ELISA kit (CE)
Features
- Working range: 2.7 ? 1000 ng/mL
- Sample: serum or EDTA plasma ? 100 ?L
- Analytical sensitivity: 2.7 ng/mL
Applications
- In liver diseases, determination of fibrosis staging, and monitoring ongoing antifibrotic therapies and outcomes
- In liver diseases, indicator of liver transplant rejection
- In joint disease, evaluation of degenerative joint disease, monitoring disease progression and therapeutic efficacy
- Tumor marker in prostate, breast and bladder cancers
- In diabetes, a high level of hyaluronic acid correlates with diabetic complications and occurrence of diabetic angiopathy
작용원리
ELISA
Microplate
히알루론산 결합 단백질은 샌드위치법에 사용된다. 히알루론산 결합 단백질은 마이크로테이터 플레이트에 코팅되어 있다. 히알루론산 결합 단백질과 결합된 Horse-radish peroxidase이 검출된다.
*3 step 검사 절차: RT(실온)에서 2시간 / Shaking + RT(실온)에서 30분 / Shaking + RT(실온)에서 30분 / Shaking.